American Heart Journal
Volume 156, Issue 6 , Pages 1201.e1-1201.e11, December 2008

Selenium supplementation improves antioxidant capacity in vitro and in vivo in patients with coronary artery disease:

The SElenium Therapy in Coronary Artery disease Patients (SETCAP) Study

  • Renate Schnabel, MD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • Both authors contributed equally.
    • ,
  • Edith Lubos, MD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
    • Both authors contributed equally.
    • ,
  • Claudia M. Messow

      Affiliations

    • The Institute of Medical Biostatistics, Epidemiology and Informatics, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Christoph R. Sinning, MD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Tanja Zeller, PhD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Philipp S. Wild, MD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Dirk Peetz, MD

      Affiliations

    • The Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Diane E. Handy, PhD

      Affiliations

    • Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
    • ,
  • Thomas Munzel, MD, FAHA

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Joseph Loscalzo, MD, PhD

      Affiliations

    • Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA
    • ,
  • Karl J. Lackner, MD

      Affiliations

    • The Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg-University, Mainz, Germany
    • ,
  • Stefan Blankenberg, MD

      Affiliations

    • Department of Medicine II, Johannes Gutenberg-University, Mainz, Germany
    • Corresponding Author InformationReprint requests: Stefan Blankenberg, MD, Department of Medicine II, Johannes Gutenberg-University, Mainz, Langenbeckstr. 1, 55131 Mainz, Germany.

Received 17 June 2008; accepted 8 September 2008.

Background

Selenium is a central determinant of antioxidative glutathione peroxidase 1 (GPx-1) expression and activity. The relevance of selenium supplementation on GPx-1 in coronary artery disease (CAD) needs to be established. We assessed the effect of selenium supplementation on GPx-1 in cell culture and on endothelial function in a prospective clinical trial.

Methods

Human coronary artery endothelial cells were incubated with 5.78 to 578 nmol/L sodium selenite, Se-methyl-selenocysteine hydrochloride, or seleno-l-methionine. Glutathione peroxidase 1 mRNA and protein expression and activity were measured. Coronary artery disease patients (n = 465) with impaired endothelial function (flow-mediated dilation [FMD] <8%) were randomly assigned to receive 200 or 500 μg sodium selenite daily or matching placebo during a 12-week period. We tested the effect on red blood cell GPx-1 activity and brachial artery FMD. Furthermore, differences in biomarkers of oxidative stress and inflammation were measured.

Results

Sodium selenite and Se-methyl-selenocysteine hydrochloride increased GPx-1 protein and activity in a dose-dependent manner (P < .0001). The intention-to-treat groups comprised 433 CAD patients. Glutathione peroxidase 1 activity increased from 37.0 U/gHb (31.3-41.7) to 41.1 U/gHb (35.2-48.4) (P < .0001) in the 200 μg and from 38.1 U/gHb (33.2-43.8) to 42.6 U/gHb (35.0-49.1) (P < .0001) in the 500 μg sodium selenite group treated for 12-weeks. No relevant changes were observed for FMD or biomarkers of oxidative stress and inflammation.

Conclusions

Sodium selenite supplementation increases GPx-1 activity in endothelial cells and in CAD patients. Future studies have to demonstrate whether long-term CAD outcome can be improved.

 

 The study was funded by an unrestricted grant from Biosyn Arzneimittel GmbH, Fellbach, Germany; by National Institutes of Health (NIH) (Bethesda, MD) grants HL 61795, HV 28178, and HL 81587 from the National Heart, Lung, and Blood Institute (J.L.) (Bethesda, MD); and by grants from the Deutsche Forschungsgemeinschaft (Bonn, Germany) LU 1452/1-1 (E.L.) and SCHN 1149/1-1 (R.S.).

PII: S0002-8703(08)00789-8

doi:10.1016/j.ahj.2008.09.004

American Heart Journal
Volume 156, Issue 6 , Pages 1201.e1-1201.e11, December 2008

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